dc.contributor.author | Mallet, Alex | |
dc.date.accessioned | 2007-02-06T16:41:27Z | |
dc.date.available | 2007-02-06T16:41:27Z | |
dc.date.issued | 2007-02-06T16:41:27Z | |
dc.identifier.uri | http://hdl.handle.net/1721.1/35880 | |
dc.description.abstract | I performed computational analyses of various approaches to generating re-
engineered versions of the genome of bacteriophage T7. I analyzed a proposed design for a
re-engineered genome by examining conservation of T7 genes across related phages, and
looking for RNA secondary structure arising from the re-engineered genome that might
contribute to unwanted regulation. In addition, I proposed two methods of generating
libraries of T7 genomes, and implemented simulations showing that the proposed methods
are theoretically feasible. I conclude with thoughts on how to further validate my proposed
approaches to genome generation, and suggest a specific high-throughput method of
characterizing rebuilt genomes. | en |
dc.language.iso | en_US | en |
dc.subject | bacteriophage t7 | en |
dc.subject | refactoring | en |
dc.title | Analysis of Targeted and Combinatorial Approaches to Phage T7 Genome Generation | en |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | |